Disease X-19 Medical Review

medrxiv Subject Collection: Infectious Diseases: Oral colonization by Entamoeba gingivalis and Trichomonas tenax: PCR-based study in health, gingivitis, and periodontitis

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The etiology of periodontitis needs further investigation, as is the place of gingivitis in its pathophysiology. A few studies linked the oral colonization by parasites (Entamoeba gingivalis and Trichomonas tenax) to the disease and its severity. The aim of this study was to estimate the prevalence of these oral parasites among healthy individuals, gingivitis and periodontitis patients in Jordan. The study was conducted by active enrolment of participants at Jordan University Hospital. The participants answered a questionnaire that included items related to possible risk factors for periodontal disease. Saliva and dental plaque samples were collected. The detection of oral parasites was done using conventional PCR and microscopic examination of wet mounts. The study population comprised a total of 237 individuals divided into three groups: healthy (n=94), gingivitis (n=53), and periodontitis (n=90). PCR results revealed that the overall prevalence of E. gingivalis was 71.7% compared to 12.2% for T. tenax. The periodontal disease group had higher prevalence of E. gingivalis and T. tenax compared to the healthy group (p
medrxiv Subject Collection: Infectious Diseases

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